1. 

   Alizee
   Member

   Hey, I like this project, I just wanted to say Congrats and Good Luck!

   I'll talk about it in my lab, it looks like a real cheap alternative to the systems we're using now (gel box + camera + computer); The only thing is SYBR safe is more expensive than Ethidium bromide although less dangerous (even though also being a DNA intercalant)... Can the blue leds also reveal BET stained gel ? I think its absorption spectra is in the UV as well as 450-500nm so it should be fine.
   Anyway, keep up this great initiative, and all the others more and more needed in this politic-economic context where only very high and excellent achievers in research are rewarded with funds, selecting research economically viable on short term. Your initiative will help not only "biohackers" to do it themselves but also small labs struggling financially!

   Cheers,

   Alizée (PhD student in Paris, France)

   Posted 12 months ago #
2. 

   Tito
   Key Master

   Thanks for the feedback, Alizée!

   Sounds like the spectra of BET might work. Let me know if you would be interested in testing it, we can work something out :)

   Tito

   Posted 12 months ago #
3. 

   Norman
   Member

   Do you know the emission spectra of BET? It may work if it's above 510nm or so... higher the better. What are the components of this dye? Is it cyanine Cy3 or Cy5?

   Posted 12 months ago #
4. 

   Anonymous
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   Posted 8 months ago #
7. 

   Anonymous
   Unregistered

   you should not buy this product. You may have to pay Clare Chemical for license fee because Pear Biotech has not licensed the blue light transilluminator technology from Clare Chemical.

   Posted 7 months ago #
8. 

   Anonymous
   Unregistered

   I don't think there is an issue with Clare Chemical. I demoed one of their dark readers and they use a regular light bulb with blue plexiglass. They do NOT use an LED array. Now, invitrogen's unit is a blue LED array, but they are not exactly the first to develop it for this purpose. I've actually downloaded the patent applications from google.

   Here's a funny alternative, they (can't remember which company) at one point tried an LCD screen set to blue as the light source!!! Wouldn't it be cool if you could just find a small LCD? You can learn so much from looking at patents.

   Posted 7 months ago #
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    Posted 6 months ago #

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