http://www.pearlbiotech.com/discuss/ Just another bbPress community en-US Thu, 09 Sep 2010 03:47:51 +0000 http://bbpress.org/?v=1.0.1 q http://www.pearlbiotech.com/discuss/search.php http://www.pearlbiotech.com/discuss/topic.php?id=2440#post-2484 Tue, 13 Jul 2010 05:20:35 +0000 AtSPX1 2484@http://www.pearlbiotech.com/discuss/ <p>Hello,</p> <p>I am a high school senior with a Senior Thesis focused in Biochemistry. I am working with simple Peptide Nucleic Acid monomers and protecting groups to test if the protecting groups could allow synthesis and self assembly of PNA oligomers. I know you are involved in both Open Gel Box and OpenPCR, so I was wondering if you know if PNA would function the same way DNA should in both of these.</p> <p>By the way, I recommend there by an OpenHPLC at some point so I can hack together an analytic biochemistry lab.</p> <p>Thanks,<br /> Brendon </p> http://www.pearlbiotech.com/discuss/topic.php?id=2438#post-2475 Fri, 28 May 2010 21:18:02 +0000 Dr_evil288 2475@http://www.pearlbiotech.com/discuss/ <p>Out of interest, how would someone get hold the primers for a PCR? I would love you guys to get an open gene machine going! </p> <p>Cheers. </p> http://www.pearlbiotech.com/discuss/topic.php?id=2439#post-2476 Mon, 31 May 2010 18:18:50 +0000 blipton 2476@http://www.pearlbiotech.com/discuss/ <p>I saw the video,<br /> <a href="http://www.youtube.com/watch?v=zrejvnVfZJE" rel="nofollow">http://www.youtube.com/watch?v=zrejvnVfZJE</a> </p> <p>and I would love to play with it! I'm only a sophomore in high school, but I love chemistry and biology and pretty much hacking in general!</p> <p>What I'm curious though, is that with 3 billion base pairs of dna, which or how much can the device look at? </p> <p>Thanks!<br /> Ben</p> <p><a href="http://genome.ucsc.edu/cgi-bin/hgGateway" rel="nofollow">http://genome.ucsc.edu/cgi-bin/hgGateway</a><br /> <a href="http://www.geneious.com/" rel="nofollow">http://www.geneious.com/</a> </p> http://www.pearlbiotech.com/discuss/topic.php?id=2117#post-2157 Thu, 06 May 2010 14:57:18 +0000 Anonymous 2157@http://www.pearlbiotech.com/discuss/ <p>Can you just use a standard digital camera to take a picture of the gel through the orange filter? Has anybody tried this? Also, any idea if this system would work with "GelRed" <a href="http://www.biotium.com/product/price_and_info.asp?item=41003&amp;Sub_Section=09A" rel="nofollow">http://www.biotium.com/product/price_and_info.asp?item=41003&amp;Sub_Section=09A</a> . Excitation and Emission spectra are shown in link. </p> http://www.pearlbiotech.com/discuss/topic.php?id=5#post-7 Wed, 09 Sep 2009 23:08:25 +0000 Alizee 7@http://www.pearlbiotech.com/discuss/ <p>Hey, I like this project, I just wanted to say Congrats and Good Luck!</p> <p>I'll talk about it in my lab, it looks like a real cheap alternative to the systems we're using now (gel box + camera + computer); The only thing is SYBR safe is more expensive than Ethidium bromide although less dangerous (even though also being a DNA intercalant)... Can the blue leds also reveal BET stained gel ? I think its absorption spectra is in the UV as well as 450-500nm so it should be fine.<br /> Anyway, keep up this great initiative, and all the others more and more needed in this politic-economic context where only very high and excellent achievers in research are rewarded with funds, selecting research economically viable on short term. Your initiative will help not only "biohackers" to do it themselves but also small labs struggling financially!</p> <p>Cheers,</p> <p>Alizée (PhD student in Paris, France) </p> http://www.pearlbiotech.com/discuss/topic.php?id=675#post-708 Sun, 07 Feb 2010 18:03:36 +0000 Anonymous 708@http://www.pearlbiotech.com/discuss/ <p>Hey Guys,</p> <p>Absolutely fabulous product. I've been thinking about making my own but didn't know where to start. Do you guys have a parts list and instructions. I'm trying to figure out how to go from the files in the hardware section to an actual product. I mostly need the transilluminator as I already have a gel box. But what do I do with the pdf schematic? Where do I go to buy the parts, etc. </p> <p>Can someone help a newbie out???</p> <p>Thanks,</p> <p>Caroline </p> http://www.pearlbiotech.com/discuss/topic.php?id=102#post-114 Wed, 18 Nov 2009 07:12:55 +0000 derekja 114@http://www.pearlbiotech.com/discuss/ <p>Hi,</p> <p>My gelbox is on it's way! I'm hurriedly ordering some of the SYBRsafe dye as well.</p> <p>I usually use EtBr, though, and to be fair will probably still find a way to do so.</p> <p>I've got a couple of questions regarding the manufacture of the gelbox...</p> <p>1) what is the top filter? Is it comparable to the Cokin orange 002A that I would normally filter with?</p> <p>2) is there anything in the clear base that would block large portions of UV in the 312 nm range? See any problem with just sticking a UV lamp under the base rather than the blue LEDs?</p> <p>Thanks!</p> <p>--Derek </p> http://www.pearlbiotech.com/discuss/topic.php?id=40#post-46 Sun, 08 Nov 2009 11:53:39 +0000 Biotoscano 46@http://www.pearlbiotech.com/discuss/ <p>At first I would like to say CONGRATULATIONS! You've made a really great work!<br /> At second, I want to propose an improvement to the website. I think you should include some pictures with results of an experiment, just to have an idea about how is the fluorescence of the DNA bands. Maybe with another with a hand near or something that allow to know how big it's really, because in Europe, we are not familiarized whit your metric system. </p> <p>Thank you very much, Carlos Toscano from Seville (Spain) </p> http://www.pearlbiotech.com/discuss/topic.php?id=43#post-49 Mon, 09 Nov 2009 03:21:15 +0000 Biotoscano 49@http://www.pearlbiotech.com/discuss/ <p>Hi again! I would like to know how works (if it works) using the gel box with SYBR green I &amp; II and SYBR gold. Also EtBr. Because I know that absortion spectrum for all the SYBR members if very near. And with the BrEt I think that is not going to work but maybe yes or something (I don't know the spectrum for the BrEt).</p> <p>Thanks, Carlos Toscano </p> http://www.pearlbiotech.com/discuss/topic.php?id=6#post-10 Mon, 14 Sep 2009 17:23:33 +0000 wkenzie 10@http://www.pearlbiotech.com/discuss/ <p>Biotech newbie here. Am looking for a way to do my own Hep C and HIV tests on used and discarded needles and other drug paraphrenalia left in our neighbourhood. Wondering if Pearl gel box will help me in accomplishing this. If not, any pointers? Thanks. </p>