http://www.pearlbiotech.com/discuss/ Just another bbPress community en-US Fri, 30 Jul 2010 11:53:33 +0000 http://bbpress.org/?v=1.0.1 q http://www.pearlbiotech.com/discuss/search.php http://www.pearlbiotech.com/discuss/topic.php?id=2440#post-2484 Tue, 13 Jul 2010 05:20:35 +0000 AtSPX1 2484@http://www.pearlbiotech.com/discuss/ <p>Hello,</p> <p>I am a high school senior with a Senior Thesis focused in Biochemistry. I am working with simple Peptide Nucleic Acid monomers and protecting groups to test if the protecting groups could allow synthesis and self assembly of PNA oligomers. I know you are involved in both Open Gel Box and OpenPCR, so I was wondering if you know if PNA would function the same way DNA should in both of these.</p> <p>By the way, I recommend there by an OpenHPLC at some point so I can hack together an analytic biochemistry lab.</p> <p>Thanks,<br /> Brendon </p> http://www.pearlbiotech.com/discuss/topic.php?id=2438#post-2480 Wed, 02 Jun 2010 03:58:41 +0000 Tito 2480@http://www.pearlbiotech.com/discuss/ <p>Hi,<br /> I think this thread might be helpful for ya:<br /> <a href="http://www.pearlbiotech.com/discuss/topic.php?id=2439" rel="nofollow">http://www.pearlbiotech.com/discuss/topic.php?id=2439</a></p> <p>Tito </p> http://www.pearlbiotech.com/discuss/topic.php?id=2439#post-2479 Wed, 02 Jun 2010 03:48:07 +0000 Tito 2479@http://www.pearlbiotech.com/discuss/ <p>You're exactly right, primers are used to mark the DNA area of interest. These days, a company will synthesize them for you -- such as Invitrogen: <a href="https://commerce.invitrogen.com/index.cfm?fuseaction=orderAssemble.simpleAddPrimerToCart&amp;primerTypeCode=D&amp;lid=DNABasic" rel="nofollow">https://commerce.invitrogen.com/index.cfm?fuseaction=orderAssemble.simpleAddPrimerToCart&amp;primerTypeCode=D&amp;lid=DNABasic</a></p> <p>It's a bit complicated though. As a starter guide for PCR, here's a description of a kit offered by Carolina. (The chemicals/etc in the kit kind of suck, but the instructions are good)<br /> <a href="http://www.carolina.com/category/teacher+resources/instructions+and+buying+guides/biotech+kit+instruction+manuals/using+a+single-nucleotide+polymorphism+to+predict+bitter+tasting+ability.do" rel="nofollow">http://www.carolina.com/category/teacher+resources/instructions+and+buying+guides/biotech+kit+instruction+manuals/using+a+single-nucleotide+polymorphism+to+predict+bitter+tasting+ability.do</a></p> <p>And, here's a basic gel electrophoresis experiment with food coloring -- I've done it a couple times myself<br /> <a href="http://learn.genetics.utah.edu/content/labs/gel/electrophoresis/" rel="nofollow">http://learn.genetics.utah.edu/content/labs/gel/electrophoresis/</a></p> <p>The current needed is really low. I've done electrophoresis with just 4 9V batteries hooked together. It moves a bit slower than a 100V power supply, but works fine. Here's a $100 power supply to get you started with electrophoresis:<br /> <a href="http://educationalscience.com/merchant.ihtml?pid=3178&amp;step=4&amp;passwordstatus=:passwordstatus" rel="nofollow">http://educationalscience.com/merchant.ihtml?pid=3178&amp;step=4&amp;passwordstatus=:passwordstatus</a></p> <p>Let me know if I answered all your questions or if you have any others!</p> <p>Tito </p> http://www.pearlbiotech.com/discuss/topic.php?id=2439#post-2478 Tue, 01 Jun 2010 20:37:37 +0000 blipton 2478@http://www.pearlbiotech.com/discuss/ <p>Thanks, those video labs are pretty cool!</p> <p>So, if I understand correctly, the primer is what's used to mark the DNA area of interest. But practically speaking how is this done? For example, if Wiki shows a Gene with a 'UCSC' location of "Chr 7: 127.67 - 127.68 Mb", how does this get translated to the Primer (chemical) solution?</p> <p>My teacher is totally psyched about me doing this, offering whatever is in the school lab for this! However, I didn't see a parts list of required chemicals (A,C,G,T, etc). Is it common chemicals for this kind of thing? </p> <p>Also, the only power supply at school that even comes close the 100v required is a dual supply of 40v / 5A each. If I cascade (positive terminal of one, to the negative of the other) I'm thinking I can get 80V / 10A, but is that good enough? How much current at 100V is needed?</p> <p>Thanks!<br /> Ben </p> http://www.pearlbiotech.com/discuss/topic.php?id=2439#post-2477 Tue, 01 Jun 2010 00:34:53 +0000 Tito 2477@http://www.pearlbiotech.com/discuss/ <p>Hey Ben,<br /> You can look at however much of it you want. The process we showed in the video is looking at a single letter that says a lot about bitter tasting ability. With a PCR machine and a gel box, you can look at any single letter you want. Here's some great virtual labs to get you started:<br /> Extract DNA: <a href="http://learn.genetics.utah.edu/content/labs/extraction/" rel="nofollow">http://learn.genetics.utah.edu/content/labs/extraction/</a><br /> Copy DNA with PCR: <a href="http://learn.genetics.utah.edu/content/labs/pcr/" rel="nofollow">http://learn.genetics.utah.edu/content/labs/pcr/</a><br /> Visualize DNA with a gel box: <a href="http://learn.genetics.utah.edu/content/labs/gel/" rel="nofollow">http://learn.genetics.utah.edu/content/labs/gel/</a> </p> <p>Let us know what other questions you have!</p> <p>Tito<br /> Founder, Pearl Biotech </p> http://www.pearlbiotech.com/discuss/topic.php?id=2439#post-2476 Mon, 31 May 2010 18:18:50 +0000 blipton 2476@http://www.pearlbiotech.com/discuss/ <p>I saw the video,<br /> <a href="http://www.youtube.com/watch?v=zrejvnVfZJE" rel="nofollow">http://www.youtube.com/watch?v=zrejvnVfZJE</a> </p> <p>and I would love to play with it! I'm only a sophomore in high school, but I love chemistry and biology and pretty much hacking in general!</p> <p>What I'm curious though, is that with 3 billion base pairs of dna, which or how much can the device look at? </p> <p>Thanks!<br /> Ben</p> <p><a href="http://genome.ucsc.edu/cgi-bin/hgGateway" rel="nofollow">http://genome.ucsc.edu/cgi-bin/hgGateway</a><br /> <a href="http://www.geneious.com/" rel="nofollow">http://www.geneious.com/</a> </p> http://www.pearlbiotech.com/discuss/topic.php?id=2438#post-2475 Fri, 28 May 2010 21:18:02 +0000 Dr_evil288 2475@http://www.pearlbiotech.com/discuss/ <p>Out of interest, how would someone get hold the primers for a PCR? 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