Strawberries, bacteria, humans—all living things have genes, and all of these genes are made of DNA. That’s why scientists can take a gene from one living thing and put it into another. For example, they can put human genes into bacteria to make new medicines.

How do scientists take DNA out of a living thing? It’s not that hard—there are lots of ways to do it! You can follow the directions in the video below to get DNA out of a strawberry. Or you can follow the steps after that. Either way you’ll have strawberry DNA at the end!

What you need:

• measuring cup
• measuring spoons
• rubbing alcohol

• 1/2 teaspoon salt
• 1/3 cup water
• 1 tablespoon Dawn dishwashing detergent
• glass or small bowl

• cheesecloth
• funnel
• tall drinking glass
• 3 strawberries (green tops removed)
• reclosable plastic sandwich bags
• test tube or small glass jar (like the kind spices come in)
• bamboo skewer or kabob sticks  (find them at the grocery store)

What to do:

1. Chill the rubbing alcohol in the freezer. (You’ll need it later.)
2. Mix the salt, water, and Dawn detergent in a glass or small bowl. Set the mixture aside. This is your extraction liquid.
3. Line the funnel with the cheesecloth, and put the funnel’s tube into the glass.
4. Put the strawberries in the plastic bag and push out all the extra air. Seal it tightly.
5. With your fingers, squeeze and smash the strawberry mixture for 2 minutes.
6. Add 3 tablespoons of the extraction liquid you made in Step 2 to the strawberries in the bag. Push out all the extra air and reseal the bag.
7. Squeeze the strawberry mixture with your fingers for 1 minute.
8. Pour the strawberry mixture from the bag into the funnel. Let it drip into the glass until there is no liquid left in the funnel.
9. Throw away the cheesecloth and the strawberry pulp inside. Pour the contents of the glass into the test tube or small glass jar so it is 1/4 full.
10. Tilt the test tube or jar and very slowly pour the cold rubbing alcohol down the side. The alcohol should form a layer on top of the strawberry liquid. (Don’t let the alcohol and strawberry liquid mix. The DNA collects between the two layers!)
11. Dip the bamboo skewer into the test tube where the alcohol and strawberry layers meet. Pull up the skewer. The whitish, stringy stuff is DNA containing strawberry genes!

You can try these steps to purify DNA from lots of other living things. Grab some oatmeal or kiwis from the kitchen and try it again! Which foods give you the most DNA?

Here is a link to troubleshooting tips and FAQ list from the “Extract DNA from Anything Living” experiment: 20 Most Frequently Asked Questions

Syndicated from thetech.org “Do-it-yourself Strawberry DNA”

Nature this month has a wonderful piece on garage biotech, including a beautiful picture an OpenPCR prototype built by Josh Perfetto and I. Read more at: http://www.nature.com/news/2010/101006/full/467650a.html

Tito Jankowski is one of the organizers of the DIY Bio community and he’s trying to make the field of biotechnology accessible to amateurs as well. He thinks anyone should be able to look at their DNA. You can start by swabbing saliva from inside your mouth and then look at it in a small, home-based lab. His small San Francisco-based company, Pearl Biotech, is starting to develop some of the equipment you’d need. The Pearl Gel Box, a gel electrophoresis system, is based on an open-source hardware design, like many of these projects, which means that the specifications are open and shared publicly. Anyone could use these specifications to build their own version of this equipment and customize it for a specific application. Or you can buy the Pearl Gel box in versions from $189 to $500, depending on how much assembly you’re willing to do yourself. Commercial versions cost more than $1,000 but most importantly, their producers don’t expect anyone but scientists or technicians to be using them.

Read more at the MAKE Magazine Blog…